Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem

Natacha Jean (1) , Estelle Dumont (1, 2) , Gaël Durrieu (1, 3) , Thierry Balliau (1, 4) , Jean-Louis Jamet (1) , Sébastien Personnic (1, 5) , Cédric Garnier (1)

1 PROTEE - Processus de Transfert et d'Echanges dans l'Environnement - EA 3819
2 MCT - Membranes et cibles thérapeutiques
3 ONERA - The French Aerospace Lab [Toulouse]
4 GQE-Le Moulon - Génétique Quantitative et Evolution - Le Moulon (Génétique Végétale)
5 MIO - Institut méditerranéen d'océanologie

Bidimensional and monodimensional polyacrylamide gel electrophoresis were used to study protein expression from zooplankton collected in thirteen stations of Toulon Bay (NW Mediterranean). In this ecosystem, Little Bay showed higher trace metal concentrations (13.5–23.8 nM for Cu, 0.73–1.24 nM for Pb, 27.8–58.7 nM for Zn) than Large Bay (Cu 2.2–15.6 nM; Pb 0.19–0.78 nM; Zn 9.0–38.8 nM). Trace metals positively correlated (p < 0.05) with expression of four zooplankton proteins (MW in kDa/pI: 25.0/5.6; 48.8/4.1; 38.2/4.4; 38.3/5.8) and with biomass of Oithona nana, predominant copepod in Little Bay. Sequencing by LC–MS/MS putatively provided zooplankton identity of these proteins: they were cytoskeleton actin, except one protein that was the chaperone calreticulin. We suggest that actin and calreticulin could be regarded as zooplankton markers of metal stress and be involved in a possible tolerance of O. nana to contamination, contributing to its development in a marine perturbed ecosystem.

Keywords: Bidimensional polyacrylamide gel electrophoresis Monodimensional polyacrylamide gel electrophoresis; Liquid chromatography tandem mass spectrometry; Trace metals; Zooplankton; POLYACRYLAMIDE-GEL ELECTROPHORESIS; TOXIN-PRODUCING DINOFLAGELLATE; DISSOLVED ORGANIC-MATTER; PACIFIC SURFACE WATERS; HARMFUL ALGAL BLOOMS; SOUTH CHINA SEA; 2-DIMENSIONAL ELECTROPHORESIS; ALEXANDRIUM-TAMARENSE; PROTEOMIC APPROACH

Keywords: Metaproteomics; PARTICULATE PROTEINS

Domains: Analytical chemistry; Continental interfaces, environment; Ocean, Atmosphere; Geochemistry; Environmental and Society; Global Changes

Natacha Jean, Estelle Dumont, Gaël Durrieu, Thierry Balliau, Jean-Louis Jamet, et al.. Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem. Marine Environmental Research, 2012, 80, pp.12-26. ⟨10.1016/j.marenvres.2012.06.004⟩. ⟨hal-01096852⟩