Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem

Abstract : Bidimensional and monodimensional polyacrylamide gel electrophoresis were used to study protein expression from zooplankton collected in thirteen stations of Toulon Bay (NW Mediterranean). In this ecosystem, Little Bay showed higher trace metal concentrations (13.5–23.8 nM for Cu, 0.73–1.24 nM for Pb, 27.8–58.7 nM for Zn) than Large Bay (Cu 2.2–15.6 nM; Pb 0.19–0.78 nM; Zn 9.0–38.8 nM). Trace metals positively correlated (p < 0.05) with expression of four zooplankton proteins (MW in kDa/pI: 25.0/5.6; 48.8/4.1; 38.2/4.4; 38.3/5.8) and with biomass of Oithona nana, predominant copepod in Little Bay. Sequencing by LC–MS/MS putatively provided zooplankton identity of these proteins: they were cytoskeleton actin, except one protein that was the chaperone calreticulin. We suggest that actin and calreticulin could be regarded as zooplankton markers of metal stress and be involved in a possible tolerance of O. nana to contamination, contributing to its development in a marine perturbed ecosystem.
Complete list of metadatas

Cited literature [73 references]  Display  Hide  Download

https://hal-univ-tln.archives-ouvertes.fr/hal-01096852
Contributor : Cédric Garnier <>
Submitted on : Monday, January 5, 2015 - 4:59:50 PM
Last modification on : Wednesday, September 18, 2019 - 2:48:02 PM
Long-term archiving on : Monday, April 6, 2015 - 10:26:05 AM

File

P24 Jean 2012.pdf
Files produced by the author(s)

Identifiers

Collections

Citation

Natacha Jean, Estelle Dumont, Gaël Durrieu, Thierry Balliau, Jean-Louis Jamet, et al.. Protein expression from zooplankton communities in a metal contaminated NW mediterranean coastal ecosystem. Marine Environmental Research, Elsevier, 2012, 80, pp.12-26. ⟨10.1016/j.marenvres.2012.06.004⟩. ⟨hal-01096852⟩

Share

Metrics

Record views

194

Files downloads

430