Shear Stress as a Major Driver of Marine Biofilm Communities in the NW Mediterranean Sea

Elisa C P Catão , Thomas Pollet (1, 2) , Benjamin Misson (3) , Cédric Garnier (3) , Jean-François Ghiglione (4) , Raphaëlle Barry-Martinet (1) , Marine Maintenay (1) , Christine Bressy (1) , Jean-François Briand (1)

1 MAPIEM - Laboratoire Matériaux Polymères Interfaces Environnement Marin - EA 4323
2 BIPAR - Biologie moléculaire et immunologie parasitaires et fongiques
3 MIO - Institut méditerranéen d'océanologie
4 LOMIC - Laboratoire d'Océanographie Microbienne

While marine biofilms depend on environmental conditions and substrate, little is known about the influence of hydrodynamic forces. We tested different immersion modes (dynamic, cyclic and static) in Toulon Bay (north-western Mediterranean Sea; NWMS). The static mode was also compared between Toulon and Banyuls Bays. In addition, different artificial surfaces designed to hamper cell attachment (self-polishing coating: SPC; and fouling-release coating: FRC) were compared to inert plastic. Prokaryotic community composition was affected by immersion mode, surface characteristics and site. Rhodobacteriaceae and Flavobacteriaceae dominated the biofilm community structure, with distinct genera according to surface type or immersion mode. Cell density increased with time, greatly limited by hydrodynamic forces, and supposed to delay biofilm maturation. After 1 year, a significant impact of shear stress on the taxonomic structure of the prokaryotic community developed on each surface type was observed. When surfaces contained no biocides, roughness and wettability shaped prokaryotic community structure, which was not enhanced by shear stress. Conversely, the biocidal effect of SPC surfaces, already major in static immersion mode, was amplified by the 15 knots speed. The biofilm community on SPC was 60% dissimilar to the biofilm on the other surfaces and was distinctly colonized by Sphingomonadaceae ((Alter)Erythrobacter). At Banyuls, prokaryotic community structures were more similar between the four surfaces tested than at Toulon, due possibly to a masking effect of environmental constraints, especially hydrodynamic, which was greater than in Toulon. Finally, predicted functions such as cell adhesion confirmed some of the hypotheses drawn regarding biofilm formation over the artificial surfaces tested here.

Mots clés: marine microbiome; multi-species biofilm; hydrodynamic; artificial surface; Sphingomonadaceae

Domaines: Biodiversité et Ecologie; Ecosystèmes; Bio-Informatique, Biologie Systémique [q-bio.QM]

Elisa C P Catão, Thomas Pollet, Benjamin Misson, Cédric Garnier, Jean-François Ghiglione, et al.. Shear Stress as a Major Driver of Marine Biofilm Communities in the NW Mediterranean Sea. Frontiers in Microbiology, 2019, ⟨10.3389/fmicb.2019.01768⟩. ⟨hal-02269986⟩