[68Ga]Ga-FAPI-46 synthesis on a GAIA® module system: thorough study of the automated radiolabeling reaction conditions

Léa Rubira (1) , Charlotte Donzé (1) , Juliette Fouillet (1) , Benjamin Algudo (1) , Pierre Olivier Kotzki (2) , Emmanuel Deshayes (1) , Cyril Fersing (1, 3)

1 ICM - UNICANCER - Institut régional du Cancer Montpellier Val d'Aurelle
2 IRCM - U1194 Inserm - UM - Institut de Recherche en Cancérologie de Montpellier
3 IBMM - Institut des Biomolécules Max Mousseron [Pôle Chimie Balard]

The influence of several parameters involved in the 68Ga radiolabeling of FAPI-46 was studied at the scale of the automated reaction. Among the buffers tested, HEPES 0.3 M pH 4 allowed both high radiochemical purity (RCP) and radiochemical yield (RCY), without prepurification of 68Ga but after final purification of [68Ga]Ga-FAPI-46 on a C18 cartridge. A longer reaction time did not show significant benefit on the RCP, while higher loads of FAPI-46 and gentisic acid as anti-radiolysis compound allowed better RCY.

Keywords: FAPI-46; Fibroblast; Automated production; Radiochemistry; Molecular imaging; Gallium-68

Domains: Radiochemistry; Cancer; Imaging

Léa Rubira, Charlotte Donzé, Juliette Fouillet, Benjamin Algudo, Pierre Olivier Kotzki, et al.. [68Ga]Ga-FAPI-46 synthesis on a GAIA® module system: thorough study of the automated radiolabeling reaction conditions. Applied Radiation and Isotopes, 2024, 206, pp.111211. ⟨10.1016/j.apradiso.2024.111211⟩. ⟨hal-04544439⟩